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"Experientia docet" - Experience is the best teacher

Apoptosis

Hydrogen peroxide concentration and DNA fragmentation of buffalo oocytes matured in sericin­supplemented maturation medium

  • 2019
  • Issue: 2
  • Volume: 49
S. Gustina, N.W.K. Karja, H. Hasbi, M.A. Setiadi & I. Supriatna Page: 227 - 234

This study was conducted to investigate the effects of sericin on meiotic maturation, hydrogen peroxide (H2O2) concentration and deoxyribonucleic acid (DNA) fragmentation in buffalo oocytes. Oocytes were matured in vitro in tissue culture medium (TCM­199) with in vitro maturation (IVM)…

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Dose-dependent effects of luteinizing hormone and follicle stimulating hormone on in vitro maturation, apoptosis, secretion function and expression of follicle stimulating hormone receptor and luteinizing hormone receptor of sheep oocytes

  • 2018
  • Issue: 2
  • Volume: 48
S. Wei, Y. Deng, L. Lai, H. Liang & Z. Gong Page: 369 - 378

The present study compared the effects of follicle stimulating hormone (FSH) and luteinizing hormone (LH) on in vitro maturation (IVM), apoptosis, and secretion function in sheep oocytes, as well as gene expressions of the receptors (FSHR, LHR, and GnRHR) in…

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Optimization of in vitro culture and transfection condition of bovine primary spermatogonial stem cells

  • 2018
  • Issue: 1
  • Volume: 48
A. Jafarnejad, M. Aminafshar, M. Zandi, M. R. Sanjabi & N. Emamjomeh Kashan Page: 108 - 116

The present study aimed to optimize the in vitro culture and transfection efficiency of bovine primary spermatogonial stem cells (SSCs). To this end, SSCs were obtained from newborn Holstein bull calves by two-step enzymatic digestion. After enrichment and culture, SSCs…

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