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"Experientia docet" - Experience is the best teacher

Hydrogen peroxide concentration and DNA fragmentation of buffalo oocytes matured in sericin­supplemented maturation medium

  • 2019
  • Issue: 2
  • Volume: 49
S. Gustina, N.W.K. Karja, H. Hasbi, M.A. Setiadi & I. Supriatna Page: 227 - 234

This study was conducted to investigate the effects of sericin on meiotic maturation, hydrogen peroxide (H2O2) concentration and deoxyribonucleic acid (DNA) fragmentation in buffalo oocytes. Oocytes were matured in vitro in tissue culture medium (TCM­199) with in vitro maturation (IVM)…

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Gene expression and maturation evaluation of sheep oocytes cultured in medium supplemented with natural antioxidant source

  • 2018
  • Issue: 2
  • Volume: 48
I. A. H. Barakat, R. A. Alajmi, K. M. A. Zoheir, L. M. Salem & A. R. Al-Hemidiy Page: 261 - 270

Any improvements in assisted reproduction techniques at experimental level are important in in vitro production of large animal and human embryos. The in vitro embryo production system includes three major steps, namely in vitro maturation of the primary oocytes, in…

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Effects of various concentrations of gonadotropins and 17β estradiol on the in vitro maturation of cattle oocytes selected using brilliant cresyl blue staining

  • 2016
  • Issue: 3
  • Volume: 46
K.P.M. Lekola, J.W. Ng’ambi, M. Nkadimeng, M.L. Mphaphathi & T.L. Nedambale Page: 321 - 326

The objective of this study was to determine the in vitro maturation rate of cattle oocytes selected with brilliant cresyl blue (BCB) stain, in tissue culture medium 199 (TCM 199) supplemented with various concentrations of hormones. Oocytes were retrieved from…

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