Isolation of total ribonucleic acid from fresh and frozen-thawed boar semen and its relevance in transcriptome studies (Short communication)
The main objective of this study was to isolate high-quality total ribonucleic acid (RNA) from raw fresh semen and frozen-thawed boar semen, using a protocol comprising the conventional TRIzol assay and a membrane-based technique, the PureLink RNA mini kit. Bioanalyzer…