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"Experientia docet" - Experience is the best teacher

Optimization of in vitro culture and transfection condition of bovine primary spermatogonial stem cells

  • 2018
  • Issue: 1
  • Volume: 48
A. Jafarnejad, M. Aminafshar, M. Zandi, M. R. Sanjabi & N. Emamjomeh Kashan Page: 108 - 116

The present study aimed to optimize the in vitro culture and transfection efficiency of bovine primary spermatogonial stem cells (SSCs). To this end, SSCs were obtained from newborn Holstein bull calves by two-step enzymatic digestion. After enrichment and culture, SSCs…

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