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"Experientia docet" - Experience is the best teacher

viability

Optimization of in vitro culture and transfection condition of bovine primary spermatogonial stem cells

  • 2018
  • Issue: 1
  • Volume: 48
A. Jafarnejad, M. Aminafshar, M. Zandi, M. R. Sanjabi & N. Emamjomeh Kashan Page: 108 - 116

The present study aimed to optimize the in vitro culture and transfection efficiency of bovine primary spermatogonial stem cells (SSCs). To this end, SSCs were obtained from newborn Holstein bull calves by two-step enzymatic digestion. After enrichment and culture, SSCs…

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Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

  • 2017
  • Issue: 1
  • Volume: 47
A.M. Raseona, O.A. Ajao, L.D Nethengwe, L.R Madzhie, T.L. Nedambale & D.M. Barry Page: 49 - 55

The aim of this study was to evaluate the viability of bull spermatozoa diluted with commercial semen extender and two culture media stored at controlled room temperature (24 °C) for 72 hours. Two Nguni bulls were used for semen collection…

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