The aim of this study was to evaluate the extender composition, presence or absence of seminal plasma and dilution rate of spermatozoa for the preservation of fresh semen during three days before artificial insemination (AI). The percentage of kidding was influenced by the duration of storage: 4 h = 71%; 28 h = 61%; 52 h = 39%; 76 h = 28%. The fertility rate decreased in both extenders tested (milk or NPPC) when the storage duration increased. Evidence was given that the removal of seminal plasma by centrifugation at collection does not improve the fertility rate. Using the same number of total spermatozoa per straw (100 x 106), but with a variable inseminated volume of 0.20 mL or 0.40 mL per straw, AI results showed that the reduction of the concentration of spermatozoa in the AI dose did not improve the fertility rate after AI, whatever the storage duration.