This paper discusses competition for land between communal grazing livestock systems and emerging preferences for wildlife-based tourism land uses in the Greater Limpopo Transfrontier Park. Renewed efforts to improve livestock production as a tool for rural development in Southern Africa come at a time that new transfrontier parks present new opportunities for rural communities to generate incomes from tourism. These multiple opportunities for rural livelihoods intensify competing claims on grazing land, which will likely influence the nature and future of livestock production at the wildlife-based tourism/livestock interface. Data on livestock numbers, land use preferences and uses of grazing land were collected through examination of dip records, focus group discussions and structured interviews with 540 households. The data were analysed through weighted rankings, Pearson chi-square tests and general descriptive statistics. Results show increasing pressure and diversified stakeholder interests on communal grazing land and a shift in preference towards more diversified use of communal grazing land. These results highlight emerging challenges for communal grazing systems at the wildlife-based tourism/livestock interface.