The study was aimed to evaluate methods for nonsurgical castration of Black Bengal bucks by intra-testicular injection of calcium chloride, sodium chloride, citric acid solutions or sterile deionized water. Twelve healthy bucks were randomly allotted to groups A, B, C, and D, consisting of three bucks each. The local anesthetic, 2% lidocaine hydrochloride, was infused into the spermatic cord of each buck, followed by bilateral intra-testicular injections of 30% calcium chloride (CaCl2), 25% sodium chloride (NaCl), 50% citric acid (C6H8O7) solutions, and sterile deionized water dosed at 2 ml per testis in groups A, B, C and D respectively. To evaluate the efficacy of chemical agents on the inactivation of testes, clinical parameters, changes in scrotal circumference, testicular fine needle aspiration (TFNA), histopathology and serum concentration of testosterone and LH were monitored. A significant decrease in the scrotal circumference was observed between the intra-testicular injection and day 14 in all the bucks. Absence of spermatogenic cells and spermatozoa in the testicular biopsy was observed on day 14 post injection in the bucks, except for one in group C. Histopathology revealed massive destruction of seminiferous tubules and disorganization of the testicular parenchyma. Serum testosterone concentration declined significantly on day 14 compared with day 0. Consequently, the gradual elevation in serum LH concentration was significant. Thus, intra-testicular injections of CaCl2 and NaCl were more effective than C6H8O7 in inducing chemical-based nonsurgical castration.
"Experientia docet" - Experience is the best teacher