The effects of an intravenous infusion of trans-10, cis-12 or cis-9, trans-11 conjugated linoleic acid (CLA) were evaluated during two consecutive days on milk fat synthesis and fatty acid profile of milk fat in dairy goats. Neither milk yield nor milk fat content were modified by any of the CLA isomers. The concentration of each CLA was not increased after each infusion. Milk fatty acid profile was not affected by any of the tested CLA’s, except for an increase in the proportion of linoleic acid. This suggests that goats and cows respond in different manners regarding mammary metabolism of these fatty acids.