The aim of this study was to identify single nucleotide polymorphisms (SNPs) associated with genomic region underlying variation in the binomial reproductive trait ‘wet-dry’ in sheep. The wet-dry phenotype was used to represent the reproductive status of the ewes, divided into https://www.viagrapascherfr.com/viagra-en-ligne-france-pas-cher/ two categories, dry (ewes that did not lamb or that lost a lamb) and wet (ewes that had lambed and had at least one suckling lamb). Wet-dry records were obtained from smallholder farmers (n = 176) and Nortier Research Farm (n = 131) for the 2014 breeding season. Ages of the ewes ranged from 1 year to 6+ years. Data from 307 individuals were analysed, of which 172 Dorpers and 4 White Dorpers were from smallholder sheep flocks and 48 Dorpers, 46 Namaqua Afrikaners, 26 South African Mutton Merinos, 4 South African Mutton Merino x Dorper and 7 Dorper x South African Mutton Merino crossbreds were from the research farm. A logistic regression model was fitted to adjust the data for the fixed effects of farm, breed, and age of the ewe and weight at mating as a covariate. Linkage disequilibrium (LD) and inbreeding coefficient were estimated using PLINK. Association analysis was performed using the genome-wide efficient mixed-model association package (GEMMA) to determine whether any significant SNPs were associated with the wet-dry reproductive trait. The wet-dry phenotype differed significantly between the smallholder (0.63 ± 0.04) and research farm flocks (0.79 ± 0.04). Genome-wide LD across all populations was r2 = 0.36. Dorpers from the smallholder flock exhibited rapid LD decay versus the resource ovine populations. Inbreeding levels were also lower for the smallholder flock (4 ± 0.003%) versus the research flock (13 ± 0.008%). No significant SNPs were identified after correction for false discovery rate. The heritability estimate for wet-dry using SNP information was 0.24. This estimate concurs with the literature and indicates the possibility of using genomic selection to improve reproduction in smallholder sheep flocks.
Preliminary genome-wide association study for wet-dry phenotype in smallholder ovine populations in South Africa
Author: A.H. Molotsi, J.F. Taylor, S.W.P. Cloete, F. Muchadeyi, J.E. Decker, L. Sandenbergh & K. Dzama
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