Preliminary results on electrophoretic and immunoelectrophoretic fractionation of bovine muscle extract

Author: D.R. Osterhoff, I.S. Ward-Cox and G. Pieterse
Year: 1971
Issue: 1
Volume: 1
Page: 103 - 107

Details of techniques are presented of starch gel-, cellulose acetate – and immunoelectrophoresis which can be used to fractionate bovine muscle extract in such a way that the variation between individual samples can be detected qualitatively. The repeatability of results in starch gel- and cellulose acetate electrophoresis was very good whereas small variations in the technique of the immunoelectrophoresis hindered correct analysis. Later, however, clear repeatability of results could also be obtained here. The following step in this study will be the determination of the hereditary background of these fractions separated by the three techniques, and the investigation of muscle protein changes in calves with spastic paresis.


Read article