Transcervical embryo recovery in Saanen goats

Author: J.B. Lima-Verde, E.S. Lopes Junior, D.I.A. Teixeira, N.R.O. Paula, A.A. Medeiros, D. Rondina and V.J.F. Freitas
Year: 2003
Issue: 2
Volume: 33
Page: 127 - 131

In order to study the embryo recovery rate using the transcervical technique in Saanen goats raised in the tropics, 20 donors were submitted to an oestrus synchronisation treatment using intravaginal progestagen sponges for 11 days. On the ninth day of treatment, goats received intramuscular injections of 50 mg cloprostenol and six injections of porcine follicle stimulating hormone (pFSH) at 12-hour intervals. Goats in oestrus were mated by Saanen bucks. Donors received an intramuscular injection of 50 mg cloprostenol 24 hours before embryo collection. At this time, the ovaries were observed by laparoscopy to verify the ovulation rate. The embryo recovery was performed six to seven days after oestrus onset by transcervical technique. Goats in which the cervix was not penetrable were submitted to embryo recovery by laparotomy. The recovered embryos were classified according to the International Embryo Transfer Society (IETS) guidelines. Oestrus was observed in 85% of goats. A response to superovulatory treatment was observed in 65% of goats and a mean ovulation rate of 11.5 ± 6.6 was recorded. The cervix was penetrable in 61.5% of goats. In the remaining goats the embryo recovery was performed by laparotomy. The recovery rate was 53.2% and 36.9% using transcervical and surgical methods, respectively. Quanti-qualitative embryo production was similar in both techniques. Embryo recovery by the transcervical technique was shown to be an alternative for collection of embryos in Saanen goats. However, further studies should be done in order to improve both the cervix penetrability and embryo recovery rate.

Keywords: embryo recovery, goats, Saanen, superovulation, transcervical technique, tropics
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